Resource,Source,Identifier HLA-A Polyclonal Antibody,Thermo Fisher Scientific,RRID:AB_2547385 CD81 Monoclonal Antibody (M38),Thermo Fisher Scientific,RRID:AB_2532984 CD81 Monoclonal Antibody (1.3.3.22),Thermo Fisher Scientific,RRID:AB_10987151 CD63 Monoclonal Antibody (Ts63),Thermo Fisher Scientific,RRID:AB_2532983 CD63 antibody,Proteintech,RRID:AB_2783831 Goat Anti-Human IgG Fc, Multi-Species SP ads-HRP,SouthernBiotech,RRID:AB_2795580 Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads-HRP,SouthernBiotech,RRID:AB_2794190 Goat Anti-Rabbit IgG Fab, Mouse/Human SP ads-HRP,SouthernBiotech,RRID:AB_2795982 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluorâ„¢ Plus 488,Thermo Fisher Scientific,RRID:AB_2633280 Goat anti-Mouse IgG (H+L), Superclonal Recombinant Secondary Antibody, Alexa Fluorâ„¢ Plus 488,Thermo Fisher Scientific,RRID:AB_2921066 MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), eBioscience,Thermo Fisher Scientific,RRID:AB_467822 MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), Biotin, eBioscience,Thermo Fisher Scientific,RRID:AB_466872 6nm Colloidal Gold-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot) (EM Grade),Jackson ImmunoResearch Labs,RRID:AB_2338015 HEK293T,Cellosaurus,RRID:CVCL_0063 Expi293F,Cellosaurus,RRID:CVCL_D615 ImageJ,https://imagej.net/,RRID:SCR_003070