Resource,Source,Identifier GFP-avictoria,Abcam,RRID:AB_303395 HA Tag Monoclonal Antibody (2-2.2.14),Thermo Fisher Scientific,RRID:AB_10978021 Mouse IgG Isotype Control,Thermo Fisher Scientific,RRID:AB_2532951 Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse,Sigma-Aldrich,RRID:AB_477585 Mouse Anti-gamma-Tubulin Monoclonal Antibody, Unconjugated, Clone GTU-88,Sigma-Aldrich,RRID:AB_477584 SPAG6 antibody,Proteintech,RRID:AB_2194798 RSPH9 antibody,Proteintech,RRID:AB_2879240 DNALI1 antibody,Proteintech,RRID:AB_2095372 SAS-6 (91.390.21),Santa Cruz Biotechnology,RRID:AB_1128357 CP110 antibody,Proteintech,RRID:AB_10638480 CEP164 antibody,Proteintech,RRID:AB_2651175 CEP162 Polyclonal Antibody,Thermo Fisher Scientific,RRID:AB_2639671 CEP290 antibody,Proteintech,RRID:AB_10973679 Anti-RPGRIP1L Antibody,Proteintech,RRID:AB_2923607 MKS1 antibody,Proteintech,RRID:AB_10637856 MKS3 antibody,Proteintech,RRID:AB_10638441 Anti-α-Tubulin antibody,Sigma-Aldrich,RRID:AB_477593 beta Tubulin (G-8),Santa Cruz Biotechnology,RRID:AB_2210962 Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488,Thermo Fisher Scientific,RRID:AB_2535764 Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647,Thermo Fisher Scientific,RRID:AB_2535809 Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594,Thermo Fisher Scientific,RRID:AB_2535781 Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647,Thermo Fisher Scientific,RRID:AB_2535811 Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488,Thermo Fisher Scientific,RRID:AB_2534074 Goat Anti-Rat IgG (H+L) Antibody, Alexa Fluor ?? 647 Conjugated,Molecular Probes,RRID:AB_141778 Alexa Fluor 488-AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H+L) ,Jackson ImmunoResearch Labs,RRID:AB_2338058 Alexa Fluor 594-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot),Jackson ImmunoResearch Labs,RRID:AB_2338062 Alexa Fluor 647-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot),Jackson ImmunoResearch Labs,RRID:AB_2338075 RRID NOT VALID - THIS IS NOT AN ANTIBODY: DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride),Thermo Fisher Scientific,RRID:AB_2629482 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP,Thermo Fisher Scientific,RRID:AB_2533947 Anti-Rabbit IgG (H+L) Polyclonal Antibody, Horseradish Peroxidase Conjugated,Innovative Research,RRID:AB_88349 Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, HRP,Thermo Fisher Scientific,RRID:AB_2535546 HEK293T,Cellosaurus,RRID:CVCL_0063 U2OS,Cellosaurus,RRID:CVCL_0042 Clustal Omega,http://www.ebi.ac.uk/Tools/msa/clustalo/,RRID:SCR_001591 MetaMorph Microscopy Automation and Image Analysis Software,http://www.moleculardevices.com/Products/Software/Meta-Imaging-Series/MetaMorph.html,RRID:SCR_002368 sigma-tau,http://www.sigma-tau.it/eng/,RRID:SCR_000488 Proteome Discoverer,https://www.thermofisher.com/order/catalog/product/IQLAAEGABSFAKJMAUH,RRID:SCR_014477 ImageJ,https://imagej.net/,RRID:SCR_003070 MATLAB,http://www.mathworks.com/products/matlab/,RRID:SCR_001622 GraphPad Prism,http://www.graphpad.com/,RRID:SCR_002798