Resource,Source,Identifier BD Horizon™ BUV496 Rat Anti-Mouse CD3 Clone 17A2,BD Biosciences,RRID:AB_3668952 CD4,BD Biosciences,RRID:AB_2741309 Ms CD5 BV750 53-7.3 50ug,BD Biosciences,RRID:AB_2871865 BUV395 Rat Anti-Mouse CD8a,BD Biosciences,RRID:AB_2732919 Brilliant Violet 421(TM) anti-mouse/human CD11b,BioLegend,RRID:AB_11203704 Ms CD11c BUV615 N418 50ug,BD Biosciences,RRID:AB_2875243 BD Horizon™ RB545 Rat Anti-Mouse CD19 Clone 1D3,BD Biosciences,RRID:AB_3668953 APC anti-mouse CD21/CD35 (CR2/CR1),BioLegend,RRID:AB_2085160 Ms CD23 BUV737 B3B4 50ug,BD Biosciences,RRID:AB_2873931 CD25 (IL-2 Receptor α),BD Biosciences,RRID:AB_2739522 Ms CD27 BB700 LG.3A10 50ug,BD Biosciences,RRID:AB_2871394 CD43,BD Biosciences,RRID:AB_2738069 CD44 Antibody, anti-mouse, APC-Vio® 770, REAfinity™,Miltenyi Biotec,RRID:AB_2733120 PerCP/Cyanine5.5 anti-mouse CD45,BioLegend,RRID:AB_2563537 APC anti-mouse CD45,BioLegend,RRID:AB_2563540 PerCP anti-mouse CD45.2,BioLegend,RRID:AB_893349 CD45R (B220) Antibody, anti-mouse, VioBlue®,Miltenyi Biotec,RRID:AB_2733945 BD Horizon™ BUV805 Rat Anti-Mouse CD62L (L-Selectin) Clone MEL-14,BD Biosciences,RRID:AB_3668954 PE anti-mouse CD64 (FcγRI),BioLegend,RRID:AB_10613467 Ms CD69 BUV661 H1.2F3 50ug,BD Biosciences,RRID:AB_2870943 CD80 (B7-1),BD Biosciences,RRID:AB_2740537 BD OptiBuild™ RY586 Rat Anti-Mouse CD93 (Early B Lineage) Clone AA4.1,BD Biosciences,RRID:AB_3668955 BD OptiBuild™ RB744 Hamster Anti-Mouse CD95 (Fas) Clone Jo2,BD Biosciences,RRID:AB_3668956 PE/Fire(TM) 640 anti-mouse CD103 Recombinant,BioLegend,RRID:AB_2924487 BD Horizon™ RB780 Rat Anti-Mouse CD127 Clone A7R34,BD Biosciences,RRID:AB_3668957 PE/Cyanine5 anti-mouse CD138 (Syndecan-1),BioLegend,RRID:AB_2894661 CD163 Monoclonal Antibody (TNKUPJ), PE-Cyanine7, eBioscience,Thermo Fisher Scientific,RRID:AB_2815112 CD185 (CXCR5),BD Biosciences,RRID:AB_2737842 APC/Fire(TM) 810 anti-mouse CD279 (PD-1),BioLegend,RRID:AB_2910292 Ms CD304 BUV563 V46-1954 50ug,BD Biosciences,RRID:AB_2917455 Brilliant Violet 711(TM) anti-mouse CD335 (NKp46),BioLegend,RRID:AB_2563289 Spark NIR(TM) 685 anti-mouse F4/80,BioLegend,RRID:AB_2924461 BD Horizon™ RB705 Rat Anti-Mouse T- and B-Cell Activation Antigen Clone GL7,BD Biosciences,RRID:AB_3668959 I-A/I-E,BD Biosciences,RRID:AB_2738192 IgD,BD Biosciences,RRID:AB_2737944 IgM,BD Biosciences,RRID:AB_2738550 PE/Fire(TM) 810 anti-mouse Ly-6C,BioLegend,RRID:AB_3083119 Ms Ly-6G R718 1A8 50ug,BD Biosciences,RRID:AB_2916402 Brilliant Violet 570(TM) anti-mouse NK-1.1,BioLegend,RRID:AB_10896952 Alexa Fluor 647 Rat Anti-Mouse Siglec-F Antibody,BD Biosciences,RRID:AB_2687570 BD OptiBuild™ RB545 Hamster Anti-Mouse TCR β Chain Clone H57-597,BD Biosciences,RRID:AB_3668961 TruStain FcX(TM) PLUS (anti-mouse CD16/32),BioLegend,RRID:AB_2783138 Brilliant Stain Buffer Plus 1000Tst,BD Biosciences,RRID:AB_2869761 HEK293T,Cellosaurus,RRID:CVCL_0063 HAP1,Cellosaurus,RRID:CVCL_Y019 pDONR223-PXK,Addgene,RRID:Addgene_23723 pLEX305-N-dTAG-CARD8,Addgene,RRID:Addgene_169989 pLEX_305-N-dTAG,Addgene,RRID:Addgene_91797 pMD2.G,Addgene,RRID:Addgene_12259 psPAX2,Addgene,RRID:Addgene_12260 Proteome Discoverer,https://www.thermofisher.com/order/catalog/product/IQLAAEGABSFAKJMAUH,RRID:SCR_014477 Skyline,https://skyline.gs.washington.edu/labkey/project/home/software/Skyline/begin.view,RRID:SCR_014080 biomaRt,https://bioconductor.org/packages/biomaRt/,RRID:SCR_019214 GATK,https://software.broadinstitute.org/gatk/,RRID:SCR_001876 sigma-tau,http://www.sigma-tau.it/eng/,RRID:SCR_000488 C57BL/6J,Unknown,RRID:IMSR_JAX:000664