Resource,Source,Identifier BUV395 Rat Anti-Mouse CD45 Clone 30-F11 (RUO),BD Biosciences,RRID:AB_2651134 CD45,BD Biosciences,RRID:AB_2738189 CD4,BD Biosciences,RRID:AB_2739008 CD279 (PD-1),BD Biosciences,RRID:AB_2737980 CD137 (4-1BB) Monoclonal Antibody (17B5), PE-Cyanine7, eBioscience,Thermo Fisher Scientific,RRID:AB_2573398 APC anti-mouse CD153,BioLegend,RRID:AB_2562680 CD279 (PD-1) Monoclonal Antibody (J43), PE-Cyanine7, eBioscience,Thermo Fisher Scientific,RRID:AB_10853805 CD137 (4-1BB) Monoclonal Antibody (17B5), APC, eBioscience,Thermo Fisher Scientific,RRID:AB_2573162 Ms CD45.1 BUV563 A20 50ug,BD Biosciences,RRID:AB_2870815 Brilliant Violet 605™ anti-mouse CD64 (FcγRI),BioLegend,RRID:AB_2629778 CD19,BD Biosciences,RRID:AB_2738141 F4/80 Monoclonal Antibody (BM8), PE-Cyanine7, eBioscience,Thermo Fisher Scientific,RRID:AB_469653 APC anti-mouse CD88 (C5aR),BioLegend,RRID:AB_10899415 MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), APC-eFluor™ 780, eBioscience,Thermo Fisher Scientific,RRID:AB_1548783 Brilliant Violet 711(TM) anti-mouse CD11c,BioLegend,RRID:AB_2563905 PE/Cyanine7 anti-mouse CD26 (DPP-4),BioLegend,RRID:AB_2564312 Brilliant Violet 650(TM) anti-mouse/rat XCR1,BioLegend,RRID:AB_2566410 FITC anti-mouse Ly-6G,BioLegend,RRID:AB_1236494 CD3e Monoclonal Antibody (145-2C11), Biotin, eBioscience,Thermo Fisher Scientific,RRID:AB_466319 Alexa Fluor(R) 647 anti-mouse CD31,BioLegend,RRID:AB_493410 InVivoPlus anti-mouse IL-4,Bio X Cell,RRID:AB_1107707 InVivoPlus anti-mouse CD16/CD32,Bio X Cell,RRID:AB_2736987 Rat Anti-IL-2 Monoclonal Antibody, Unconjugated, Clone JES6-1A12,BD Biosciences,RRID:AB_395383 Rat Anti-IL-2 Monoclonal Antibody, Biotin Conjugated, Clone JES6-5H4,BD Biosciences,RRID:AB_395384 IFN gamma Monoclonal Antibody (XMG1.2), Biotin, eBioscience,Thermo Fisher Scientific,RRID:AB_466937 InVivoMab anti-rat Kappa Immunoglobulin Light Chain,Bio X Cell,RRID:AB_10951292 InVivoPlus anti-mouse Ly6G,Bio X Cell,RRID:AB_1107721 Cytofix/Cytoperm W/GolgiPlug Kit 250Tst,BD Biosciences,RRID:AB_2869013 NOT AN ANTIBODY Alkaline Phosphatase-Streptavidin,Jackson ImmunoResearch Labs,RRID:AB_2337239 Vector Blue Substrate Kit,Vector Laboratories,RRID:AB_2336837 Imaris,http://www.bitplane.com/imaris/imaris,RRID:SCR_007370 FlowJo,https://www.flowjo.com/solutions/flowjo,RRID:SCR_008520 Python Programming Language,http://www.python.org/,RRID:SCR_008394 GraphPad Prism,http://www.graphpad.com/,RRID:SCR_002798 R Project for Statistical Computing,http://www.r-project.org/,RRID:SCR_001905 Cell Ranger ,https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-r,RRID:SCR_017344 Seurat,https://satijalab.org/seurat/get_started.html,RRID:SCR_016341 Harmony,https://github.com/immunogenomics/harmony,RRID:SCR_022206 clusterProfiler,http://bioconductor.org/packages/release/bioc/html/clusterProfiler.html,RRID:SCR_016884 ComplexHeatmap,https://bioconductor.org/packages/release/bioc/html/ComplexHeatmap.html,RRID:SCR_017270 CellChat,https://github.com/sqjin/CellChat,RRID:SCR_021946 ImmunoSpot Software for Analyzing ELISPOT Assays,http://www.immunospot.com/index.php?id=77,RRID:SCR_011082 Cornell University BRC Imaging Core Facility,https://www.biotech.cornell.edu/core-facilities-brc/facilities/imaging-facility,RRID:SCR_021741 Cornell University BRC Flow Cytometry Core Facility,https://www.biotech.cornell.edu/core-facilities-brc/facilities/flow-cytometry-facility,RRID:SCR_021740