Resource,Source,Identifier Brilliant Violet 650(TM) anti-human CD197 (CCR7),BioLegend,RRID:AB_2562041 Mouse Anti-CD107a Monoclonal Antibody, FITC Conjugated, Clone H4A3,BD Biosciences,RRID:AB_396134 CD137 Antibody, anti-human, Vio® Bright FITC, REAfinity™,Miltenyi Biotec,RRID:AB_2654984 PerCP anti-human CD14,BioLegend,RRID:AB_2876693 CD19 Antibody, anti-human, APC, REAfinity™,Miltenyi Biotec,RRID:AB_2726195 PerCP anti-human CD19,BioLegend,RRID:AB_893272 CD20 Antibody, anti-human, APC, REAfinity™,Miltenyi Biotec,RRID:AB_2656066 CD3 Antibody, anti-human, APC-Vio® 770, REAfinity™,Miltenyi Biotec,RRID:AB_2725964 CD4 Antibody, anti-human, PE, REAfinity™,Miltenyi Biotec,RRID:AB_2726036 CD4 Antibody, anti-human, PerCP-Vio® 700, REAfinity™,Miltenyi Biotec,RRID:AB_2726039 Mouse Anti-CD4 Monoclonal Antibody, APC-Cy7 Conjugated, Clone RPA-T4,BD Biosciences,RRID:AB_396913 CD8 Antibody, anti-human, VioGreen™, REAfinity™,Miltenyi Biotec,RRID:AB_2659241 CD8 Antibody, anti-human, PerCP-Vio® 700, REAfinity™,Miltenyi Biotec,RRID:AB_2659249 Anti-CD8 Antibody,Beckman Coulter,RRID:AB_1575980 Brilliant Violet 605(TM) anti-human CD8a,BioLegend,RRID:AB_2563185 CD45 Antibody, anti-human, VioGreen™, REAfinity™,Miltenyi Biotec,RRID:AB_2658245 Mouse Anti-CD45RA Monoclonal Antibody, FITC Conjugated, Clone HI100,BD Biosciences,RRID:AB_395879 Alexa Fluor® 700 anti-human IFN-γ,BioLegend,RRID:AB_961353 Mouse Anti-Human IFN-g (Interferon-gamma) Monoclonal Antibody, Unconjugated, Clone 1-D1K,MABTECH,RRID:AB_907282 Mouse Anti-Human IFN-g (Interferon-gamma) Monoclonal Antibody, Biotin Conjugated, Clone 7-B6-1,MABTECH,RRID:AB_907273 HLA-A2 Antibody, anti-human, PE, REAfinity™,Miltenyi Biotec,RRID:AB_2727641 Pacific Blue™ anti-human TNF-α,BioLegend,RRID:AB_528965 PE/Cyanine7 anti-human IL-2,BioLegend,RRID:AB_2125593 APC anti-human CD154,BioLegend,RRID:AB_314833 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) Clone 2.4G2,BD Biosciences,RRID:AB_394656 Rat Anti-CD16 / CD32 Monoclonal Antibody, Unconjugated, Clone 2.4G2,BD Biosciences,RRID:AB_394657 CD45 Antibody, anti-mouse, PerCP-Vio® 700, REAfinity™,Miltenyi Biotec,RRID:AB_2658233 APC/Cyanine7 anti-mouse TCR β chain,BioLegend,RRID:AB_893624 CD11c Antibody, anti-mouse, FITC, REAfinity™,Miltenyi Biotec,RRID:AB_2654706 CD11b Antibody, anti-mouse, VioGreen™, REAfinity™,Miltenyi Biotec,RRID:AB_2726328 INVALID RRID; DO NOT USE - BD Horizon™ BV421 Streptavidin,BD Biosciences,RRID:AB_2869475 SAV BV786 100ug,BD Biosciences,RRID:AB_2869529 Anti-FITC MicroBeads,Miltenyi Biotec,RRID:AB_244371 INVALID RRID - DO NOT USE - NOT AN ANTIBODY: Protein Transport Inhibitor Soln 0.7mL,BD Biosciences,RRID:AB_2869012 8-Color Immunophenotyping Kit, anti-human, REAfinity™,Miltenyi Biotec,RRID:AB_3096888 Mouse ELISpot IFN-γ,MABTECH,RRID:AB_3096885 Cytofix/Cytoperm Soln Kit,BD Biosciences,RRID:AB_2869008 T2,Cellosaurus,RRID:CVCL_2211 FlowJo,https://www.flowjo.com/solutions/flowjo,RRID:SCR_008520 GraphPad Prism,http://www.graphpad.com/,RRID:SCR_002798 BD LSRFortessa Cell Analyzer,https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-cell-analyzers/lsrfort,RRID:SCR_018655 Miltenyi Bioscience MACSQuant Analyzer 10,https://www.miltenyibiotec.com/CN-en/products/macs-flow-cytometry/flow-cytometers/macsquant-analyzer,RRID:SCR_020268 Miltenyi Bioscience MACSQuant Analyzer 16,https://www.miltenyibiotec.com/CN-en/products/macsquant-analyzer-16.html,RRID:SCR_020263 AID iSpot EliSpot FluoroSpot Plate Reader,https://www.aid-diagnostika.com/en/reader-systems/aid-elispot-reader/aid-ispot,RRID:SCR_025287 NC-250 Automated Cell Analyzer,https://chemometec.com/nucleocounters/nc-250/,RRID:SCR_025290 ImmunoSpot Software for Analyzing ELISPOT Assays,http://www.immunospot.com/index.php?id=77,RRID:SCR_011082