Resource,Source,Identifier beta Actin antibody - Loading Control,Abcam,RRID:AB_2305186 Anti-UBA2 polyclonal antibody,Atlas Antibodies,RRID:AB_2677479 Rabbit Anti-UBE2I / UBC9 Monoclonal Antibody, Unconjugated, Clone EP2938Y,Abcam,RRID:AB_1310787 Sumo 1 antibody [Y299],Abcam,RRID:AB_778173 Sumo 1 antibody [EP298],Abcam,RRID:AB_11156108 Sumo 2+3 antibody [8A2],Abcam,RRID:AB_1658424 Mouse Anti-polyHistidine Monoclonal Antibody, Unconjugated, Clone HIS-1,Sigma-Aldrich,RRID:AB_260015 Monoclonal ANTI-FLAG® M2 antibody produced in mouse,Sigma-Aldrich,RRID:AB_262044 Anti-GFP,Roche,RRID:AB_390913 Anti-gamma H2A.X (phospho S139) antibody,Abcam,RRID:AB_303388 alpha Tubulin (B-7),Santa Cruz Biotechnology,RRID:AB_628411 CENPA Monoclonal Antibody (3-19),Thermo Fisher Scientific,RRID:AB_2078763 NuMA (F-11),Santa Cruz Biotechnology,RRID:AB_10846197 Vinculin antibody [EPR8185],Abcam,RRID:AB_11144129 Rabbit Anti-Pericentrin Polyclonal Antibody, Unconjugated,Abcam,RRID:AB_304461 alpha Tubulin Antibody,Novus,RRID:AB_521686 Phospho-Histone H3 (Ser10) Monoclonal Antibody (K.872.3),Thermo Fisher Scientific,RRID:AB_11008586 Donkey Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 488 Conjugated,Molecular Probes,RRID:AB_141607 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488,Thermo Fisher Scientific,RRID:AB_2535792 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555,Thermo Fisher Scientific,RRID:AB_2536180 Donkey Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor ?? 555 Conjugated,Molecular Probes,RRID:AB_162543 Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555,Thermo Fisher Scientific,RRID:AB_2535855 Rabbit Anti-Mouse Immunoglobulins,Agilent,RRID:AB_2687969 Swine anti-rabbit Ig HRP,Agilent,RRID:AB_2728719 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-30,Rockland,RRID:AB_2610849 U2OS,Cellosaurus,RRID:CVCL_0042 pcDNA3/UBC9,Addgene,RRID:Addgene_20082 sigma-tau,http://www.sigma-tau.it/eng/,RRID:SCR_000488 ImageJ,https://imagej.net/,RRID:SCR_003070