BIORXIVbioRxivbioRxivCold Spring Harbor Laboratory10.64898/2026.03.10.7107601Regular Articlenew resultsmolecular biologyEvolution of Origin Sequence and Recognition for Licensing of Eukaryotic DNA ReplicationpCfB6677AddgeneRRID:Addgene_106155PlasmidspCfB8742AddgeneRRID:Addgene_126908PlasmidspSCARS1AddgeneRRID:Addgene_174868PlasmidspET28a-LICAddgeneRRID:Addgene_26094PlasmidspET28b-XIAddgeneRRID:Addgene_157988PlasmidsSAMTOOLShttp://htslib.org/RRID:SCR_002105Software and AlgorithmsDeeptoolshttps://deeptools.readthedocs.io/en/develop/RRID:SCR_016366Software and AlgorithmsHOMERhttp://homer.ucsd.edu/RRID:SCR_010881Software and Algorithmstensorflowhttps://www.tensorflow.org/RRID:SCR_016345Software and AlgorithmscryoSPARChttps://cryosparc.com/RRID:SCR_016501Software and AlgorithmsCoothttp://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/RRID:SCR_014222Software and AlgorithmsMolProbityhttp://molprobity.biochem.duke.eduRRID:SCR_014226Software and AlgorithmsPhenixhttps://www.phenix-online.org/RRID:SCR_014224Software and AlgorithmsPyMOLhttp://www.pymol.org/RRID:SCR_000305Software and Algorithms