Resource,Source,Identifier Anti-NUP133 antibody [EPR10808(B)],Abcam,RRID:AB_3711439 Mouse Anti-Nucleoporin p62 Monoclonal Antibody, Unconjugated, Clone 53,D Biosciences,RRID:AB_397863 RanBP2 antibody,bcam,RRID:AB_1142517 Rabbit anti-NUP85 Antibody, Affinity Purified,ethyl,RRID:AB_2620326 Pericentrin 1 (D-4),anta Cruz Biotechnology,RRID:AB_10988263 Nup93 (E-8),anta Cruz Biotechnology,RRID:AB_10990113 POM121 antibody [N2N3],eneTex,RRID:AB_10732546 CRM1 Antibody,ovus,RRID:AB_2215823 NTF97/Importin beta antibody [3E9],bcam,RRID:AB_2133989 SUN2 antibody [EPR6557],bcam,RRID:AB_10972497 Anti-Lamin B1 antibody - Nuclear Envelope Marker,bcam,RRID:AB_443298 Recombinant Anti-Nesprin1/Syne-1 antibody [EPR14196],bcam,RRID:AB_2917992 TMPO/LAP2 antibody,roteintech,RRID:AB_2205440 Anti-HA High Affinity; Rat monoclonal antibody (clone 3F10),oche,RRID:AB_390918 Monoclonal Anti-β-Actin antibody produced in mouse,igma-Aldrich,RRID:AB_476697 Mouse Anti-FXR1 Monoclonal Antibody, Unconjugated,illipore,RRID:AB_10806485 Anti-α-Tubulin antibody,igma-Aldrich,RRID:AB_477593 Rabbit Anti-LC3 Polyclonal, Unconjugated,ovus,RRID:AB_791015 LC3A Antibody - BSA Free,ovus,RRID:AB_10001955 SQSTM1 / P62 antibody [N3C1], Internal,eneTex,RRID:AB_2038029 Rabbit Anti-Aurora B Polyclonal Antibody, Unconjugated,bcam,RRID:AB_302923 CLIMP-63, mAb (G1/296),nzo Life Sciences,RRID:AB_2276276 CKAP4 antibody,roteintech,RRID:AB_2276275 KTN1 antibody,roteintech,RRID:AB_10642436 ATL3 antibody,roteintech,RRID:AB_2290228 Goat Anti-Mouse IgG antibody (HRP),eneTex,RRID:AB_10618076 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647,hermo Fisher Scientific,RRID:AB_2535805 Goat Anti-Mouse IgG (H+L) Highly Cross-adsorbed Antibody, Alexa Fluor ?? 568 Conjugated,olecular Probes,RRID:AB_144696 Goat Anti-Mouse IgG (H+L) Highly Cross-adsorbed Antibody, Alexa Fluor ?? 555 Conjugated,olecular Probes,RRID:AB_141780 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647,hermo Fisher Scientific,RRID:AB_2535813 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568,hermo Fisher Scientific,RRID:AB_10563566 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF™ 680 antibody produced in goat,igma-Aldrich,RRID:AB_2819153 DLD-1,Cellosaurus,RRID:CVCL_0248 HeLa,Cellosaurus,RRID:CVCL_0030 U2OS,Cellosaurus,RRID:CVCL_0042 MRC-5,Cellosaurus,RRID:CVCL_0440 NLS-mCherry-LEXY (pDN122),Addgene,RRID:Addgene_72655 mEos2-N1,Addgene,RRID:Addgene_54662 ImageJ,https://imagej.net/,RRID:SCR_003070 python-bx,https://github.com/bxlab/bx-python,RRID:SCR_024202 Adobe Illustrator,http://www.adobe.com/products/illustrator.html,RRID:SCR_010279